Revised Guidance Document 150 on Standardised Test Guidelines for Evaluating Chemicals for Endocrine Disruption

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This guidance document was originally published in 2012 and updated in 2018 to reflect new and updated OECD test guidelines, as well as reflect on scientific advances in the use of test methods and assessment of the endocrine activity of chemicals. The document is intended to provide guidance for evaluating chemical using standardised test guidelines. Specific objectives include providing a description of the OECD conceptual framework for evaluating chemicals for endocrine disruption, background on the standardised test methods used, and guidance for interpreting the outcome of individual tests. The general approach taken by the document is primarily to provide guidance on how test results might be interpreted based on the outcome of standardised assays. Key questions addressed in the document concern likely mechanisms of endocrine action and any resulting apical effects that can be attributed to such action. The document is not proscriptive but provides suggestions for possible next steps in testing (if any) which might be appropriate for a regulatory authority to take, given the various data scenarios. The guidance document is focused primarily on endocrine modalities included in the conceptual framework; estrogen, androgen, and thyroid mediated endocrine disruption and chemicals that interfer with steroidogenesis.


Avian Reproduction Test (OECD TG 206)

TG 206 is an OECD validated test to determine the effects on the reproduction of a chemical administered to birds. Birds are fed a diet containing the test chemical in various concentrations for a duration of not less than 20 weeks. At least three dietary concentrations of the test chemical are required. The maximum recommended test concentration is 1000 ppm. Birds may be kept in pens as pairs (at least 12 pens per test group) or as groups of one male and two or three females (at least 8-12 pens per group). Birds are induced, by photoperiod manipulation, to lay eggs. Eggs are collected over a ten-week period, artificially incubated and hatched, and the young maintained for 14 days. Suitable facilities for rearing birds, preferably indoors, are necessary. Mortality of adults, egg production, cracked eggs, egg shell thickness (at least two eggs from each pen), viability, hatchability and effects on young birds are observed during the study.


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