The global agro-food supply chain is controlled by a small number of large enterprises, fewer than half of which have activities on site in Africa. Within the continent, a greater number of local enterprises shape the agro-food sector. Based on corporate rankings provided by Fortune Global 500 and Jeune Afrique Les 500 published in 2007, this chapter draws a map of the corporate landscape of the major agro-food-related enterprises in Africa, both foreign and domestic, and discusses broad trends. The corporate behaviour of these large players varies across regions. The majority of firms tend to concentrate their activities in the Northern and Southern regions of the continent. However, several individual countries in other regions also show significant potential in the agro-food sector. Countries attracting the biggest number of companies include South Africa, Morocco and Nigeria. The size of target economies matters. In some sub-sectors, the integration of African agro-food enterprises into the global supply chain has begun its timid climb. Initial stages of collaboration between foreign and local firms are notable in the beverage and tobacco industries. In other sectors, African enterprises have started to seek opportunities beyond their domestic frontiers and even outside the continent. This chapter attempts to fill the gap in empirical work on agro-food enterprises in Africa and to provide a picture of the continent’s corporate map.