OECD Guidelines for the Testing of Chemicals, Section 4

Health Effects

ISSN :
2074-5788 (online)
DOI :
10.1787/20745788
Hide / Show Abstract

The OECD Guidelines for the Testing of Chemicals is a collection of about 100 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of new and existing chemical substances, chemical preparations and chemical mixtures. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification and chemical registration. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers health effects.

Also available in: French
 
Test No. 479: Genetic Toxicology: In vitro Sister Chromatid Exchange Assay in Mammalian Cells

Test No. 479: Genetic Toxicology: In vitro Sister Chromatid Exchange Assay in Mammalian Cells You or your institution have access to this content

Click to Access: 
Author(s):
OECD
Publication Date :
23 Oct 1986
Pages :
5
ISBN :
9789264071384 (PDF)
DOI :
10.1787/9789264071384-en

Hide / Show Abstract

The sister chromatid exchange (SCE) assay is a short-term test for the detection of reciprocal exchanges of DNA between two sister chromatids of a duplicating chromosome.

Detection of SCEs requires some means of differentially labeling sister chromatids, which can be achieved e.g. by incorporation of bromodeoxyuridine (BrdU) into chromosomal DNA for two cell cycles. SCEs can be measured in mammals as well as in non-mammalian systems. The test substance should be solid, liquid, vapour or gaseous. Mammalian cells in vitro are exposed to the test chemical with and without an exogenous mammalian metabolic activation system, and cultured for two rounds of replication in BrdU containing medium. At least three adequately spaced concentrations of the test substance should be used. After treatment with a spindle inhibitor to accumulate cells in a metaphase-like stage of mitosis (c-metaphase), cells are harvested and chromosome preparations are made.

NB. This Test Guideline will be deleted on 2nd April 2014.

Also available in: French