OECD Guidelines for the Testing of Chemicals, Section 4

Health Effects

ISSN :
2074-5788 (online)
DOI :
10.1787/20745788
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The OECD Guidelines for the Testing of Chemicals is a collection of about 100 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of new and existing chemical substances, chemical preparations and chemical mixtures. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification and chemical registration. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers health effects.

Also available in: French
 
Test No. 477: Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster

Test No. 477: Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster You or your institution have access to this content

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Author(s):
OECD
Publication Date :
04 Apr 1984
Pages :
6
ISBN :
9789264071346 (PDF)
DOI :
10.1787/9789264071346-en

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Mutations in the X-chromosome of Drosophila melanogaster are phenotypically expressed in males carrying the mutant gene. When the mutation is lethal in the hemizygous condition, its presence is inferred from the absence of one class of male offspring out of the two that are normally produced by a heterozygous female.

Wild-type males are treated, and mated to appropriate females. The Administration may be oral (solid or liquid), by injection or by exposure to gases or vapours. The numbers of exposure depend on whether the test is: for initial assessment of mutagenicity one exposure or for verification at least three exposure levels. The first generation female is mated individually to their brothers, and in the second generation, the progeny from each separate cross are scored for phenotypically wild-type males. Absence of these males indicates that a sexlinked recessive lethal mutation has occurred in a germ cell of the parent generation male.

NB. This Test Guideline will be deleted on 2nd April 2014.

Also available in: French