OECD Guidelines for the Testing of Chemicals, Section 4

Health Effects

English
ISSN: 
2074-5788 (online)
http://dx.doi.org/10.1787/20745788
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The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers health effects.

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Test No. 458: Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals

Test No. 458: Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals You or your institution have access to this content

English
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    http://oecd.metastore.ingenta.com/content/9716131e.pdf
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Author(s):
OECD
29 July 2016
Pages:
26
ISBN:
9789264264366 (PDF)
http://dx.doi.org/10.1787/9789264264366-en

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This Test Guideline describes an in vitro assay providing the methodology of Stably Transfected Transactivation to detect Androgen Receptor Agonists and Antagonists (AR STTA assays). The TA assay using a reporter gene technique is an in vitro tool that provides mechanistic data. The assay is used to establish signal activation or blocking of the androgen receptor caused by a ligand. Some chemicals may, in a cell type-dependent manner, display both agonist and antagonist activity and are known as selective androgen receptor modulators. Following the ligand binding, the receptor-ligand complex translocates to the nucleus where it binds specific DNA response elements and transactivates a firefly luciferase reporter gene, resulting in an increased cellular expression of the luciferase enzyme. Luciferin is a substrate that is transformed by the luciferase enzyme to a bioluminescence product that can be quantitatively measured with a luminometer. Luciferase activity can be evaluated quickly and inexpensively with a number of commercially available test kits. The test system provided in this Test Guideline utilises the AR-EcoScreenTM cell line.

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