OECD Guidelines for the Testing of Chemicals, Section 3

Environmental fate and behaviour

2074-577x (online)
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The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers environmental fate and behaviour. In 2017, the section 3 “Degradation and Accumulation” was renamed to “ Environmental fate and behaviour”  to take into account Test Guidelines measuring endpoints such as dispersion, aggregation.

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Test No. 307: Aerobic and Anaerobic Transformation in Soil

Test No. 307: Aerobic and Anaerobic Transformation in Soil You or your institution have access to this content

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24 Apr 2002
9789264070509 (PDF)

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The method described in this Test Guideline is designed for evaluating aerobic and anaerobic transformation of chemicals in soil. The experiments are performed to determine the rate of transformation of the test substance, and the nature and rates of formation and decline of transformation products, to which plants and soil organisms may be exposed.

About 50 to 200 g soil samples (a sandy loam or silty loam or loam or loamy sand) are treated with the test substance and incubated in the dark, in biometer-type flasks or in flow-through systems under controlled laboratory conditions. The treatment rate should correspond to the highest application rate of a crop protection product recommended in the use instructions. Also untreated soil samples are incubated under test conditions. These samples are used for biomass measurements during and at the end of the studies. The rate and pathway studies should normally not exceed 120 days. Duplicate incubation flasks are removed at appropriate time intervals and the soil samples extracted with appropriate solvents, of different polarity, and analysed for the test substance and/or transformation products. Volatile products are also collected for analysis using appropriate adsorption devices. Using 14C-labelled material, the various mineralisation rates of the test substance can be measured by trapping evolved 14CO2 and a mass balance, including the formation of soil bound residues, can be established.

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