OECD Guidelines for the Testing of Chemicals, Section 2

Effects on Biotic Systems

2074-5761 (online)
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The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers effects on biotic systems.

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Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure

Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure You or your institution have access to this content

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26 July 2013
9789264203723 (PDF)

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This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical.

On day 1 (D1) of the study, first instar synchronised larvae are taken from the comb of three colonies (thirty-six larvae in total per group) and individually placed into 48 well-plates where they are fed a standardized amount of artificial diet. On day 4 (D4) of the test, a single dose of the test chemical is administered to the larvae with the diet. Each group of thirty-six larvae is administered a given dose in a range of five increasing doses. Mortalities are recorded on D5, D6, and D7 of the test. The 72-hr LD50 is calculated for larvae (cumulative mortality at D7). The study report also includes a number of important elements, in particular regarding test conditions (e.g., temperature and humidity).

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