OECD Guidelines for the Testing of Chemicals, Section 2

Effects on Biotic Systems

English
ISSN: 
2074-5761 (online)
http://dx.doi.org/10.1787/20745761
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The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers effects on biotic systems.

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Test No. 231: Amphibian Metamorphosis Assay

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Test No. 231: Amphibian Metamorphosis Assay You or your institution have access to this content

English
Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723101e.pdf
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Author(s):
OECD
08 Sep 2009
Pages:
33
ISBN:
9789264076242 (PDF)
http://dx.doi.org/10.1787/9789264076242-en

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This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is recommended for use in the Guideline. The assay uses three test chemical concentrations and the necessary controls, including a carrier control if necessary. The assay starts with tadpoles at the development stage 51 on the Nieuwkoop and Faber scale and is extended for a duration of 21 days. Four replicate test vessels are used for each treatment level and control(s). After 7 days of exposure, a sub-set of tadpoles from each treatment level is sampled for the measurement of the length of the hind-limb. At termination of 21-day exposure period, developmental stage, snout-to-vent length and hind limb length are measured on all remaining tadpoles. A sub-set of tadpoles from each treatment level is fixed (whole-body or dissected) for histopathology of the thyroid gland.

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