OECD Guidelines for the Testing of Chemicals, Section 2

Effects on Biotic Systems

2074-5761 (online)
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The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers effects on biotic systems.

Also available in French
Test No. 210: Fish, Early-Life Stage Toxicity Test

Test No. 210: Fish, Early-Life Stage Toxicity Test You or your institution have access to this content


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The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested.

The early-life stages of fish are exposed to ,at least, five concentrations of the test substance dissolved in water, preferably under flow-through conditions, or where appropriate, semi-static conditions. The test starts with placing fertilised eggs (at least 60) in the test chambers and continues at least until all the control fishes are free-feeding. Lethal and sub-lethal effects are assessed and compared with control values to determine the lowest observed effect concentration and the no observed effect concentration. The study report should include measurement of the concentrations of the test substance in water at regular intervals (five at least), the dissolved oxygen, pH, total hardness and salinity, fish weight and length, as well as the observations of abnormal appearance, abnormal behaviour, hatching and survival.

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