OECD Guidelines for the Testing of Chemicals, Section 2

Effects on Biotic Systems

2074-5761 (online)
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The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers effects on biotic systems.

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Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test

Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test You or your institution have access to this content

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17 Aug 2006
9789264070066 (PDF)

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This Test Guideline is designed to assess effects on seedling emergence and early growth of higher plants following exposure to the test substance applied to the soil surface or into the soil.

Seeds are placed in contact with soil treated with the test substance and evaluated for effects following usually 14 to 21 days after 50 % emergence of the seedlings in the control group. Endpoints measured are visual assessment of seedling emergence, biomass measurements, shoot height, and the visible detrimental effects on different parts of the plant. The test can be conducted in order to determine the dose-response curve, or at a single concentration/rate as a limit test, according to the aim of the study. An appropriate statistical analysis is used to obtain effective concentration ECx or effective application rate ERx for the most sensitive parameter(s) of interest. Also, the no observed effect concentration (NOEC) and lowest observed effect concentration (LOEC) can be calculated in this test.

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