OECD Guidelines for the Testing of Chemicals, Section 1

Physical-Chemical properties

2074-5753 (online)
Hide / Show Abstract

The OECD Guidelines for the Testing of Chemicals is a collection of about 150 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification, chemical registration and in chemical evaluation. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers physical-chemical properties.

Also available in French
Test No. 123: Partition Coefficient (1-Octanol/Water): Slow-Stirring Method

Test No. 123: Partition Coefficient (1-Octanol/Water): Slow-Stirring Method You or your institution have access to this content

Click to Access: 
  • PDF
  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-123-partition-coefficient-1-octanol-water-slow-stirring-method_9789264015845-en
  • READ
11 July 2006
9789264015845 (PDF)

Hide / Show Abstract

This Test Guideline describes the slow-stirring method, which permits the determination of the 1-octanol/water partition coefficient (POW) values up to a log POW of 8.2. The partition coefficient between water and 1-octanol (POW) is defined as the ratio of the equilibrium concentrations of the pure test substance in 1-octanol saturated with water (CO) and water saturated with 1-octanol (CW).

In order to determine the partitioning coefficient, water, 1-octanol, and the test substance are equilibrated with each other at constant temperature in a thermostated stirred reactor at 25 °C and protected from daylight. Exchange between the phases is accelerated by stirring. The concentrations of the test substance in the two phases are determined. Each POW determination has to be performed employing at least three independent slow-stirring experiments with identical conditions. The regression used to demonstrate attainment of equilibrium should be based on the results of at least four determinations of CO / CW at consecutive time points.

Also available in French
loader image
Visit the OECD web site