OECD Guidelines for the Testing of Chemicals, Section 1

Physical-Chemical properties

ISSN :
2074-5753 (online)
DOI :
10.1787/20745753
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The OECD Guidelines for the Testing of Chemicals is a collection of about 100 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of new and existing chemical substances, chemical preparations and chemical mixtures. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification and chemical registration. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers physical-chemical properties.

Also available in: French
 
Test No. 117: Partition Coefficient (n-octanol/water), HPLC Method

Test No. 117: Partition Coefficient (n-octanol/water), HPLC Method You or your institution have access to this content

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Author(s):
OECD
Publication Date :
23 Nov 2004
Pages :
11
ISBN :
9789264069824 (PDF)
DOI :
10.1787/9789264069824-en

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This Test Guideline describes the HPLC method, which permits to determine the partition coefficient (n-octanol/water) (Pow).

Reverse phase HPLC is performed on analytical columns packed with a solid phase containing long hydrocarbon chains chemically bound onto silica. The chemicals are retained in the column in proportion to their hydrocarbon-water partition coefficient, with hydrophilic chemicals eluted first and lipophilic chemicals last. The HPLC method covers log Pow in the range of 0 to 6, but it can be expanded to cover the log Pow range between 6 and 10 in exceptional cases. The HPLC operation mode is isocratic. The test substance is injected in the smallest detectable quantities in the column. The retention time is determined in duplicate. The partition coefficient of the test substance is obtained by interpolation of the calculated capacity factor on the calibration graph. For very low and very high partition coefficients extrapolation is necessary.

Also available in: French