OECD Guidelines for the Testing of Chemicals, Section 3

Degradation and Accumulation

Test No. 305: Bioaccumulation in Fish: Aqueous and Dietary Exposure
ISSN :
2074-577x (online)
DOI :
10.1787/2074577x
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The OECD Guidelines for the Testing of Chemicals is a collection of about 100 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of new and existing chemical substances, chemical preparations and chemical mixtures. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification and chemical registration. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers degradation and accumulation.

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Mark Number Date TitleClick to Access
    02 Oct 2012 Click to Access:  Test No. 305: Bioaccumulation in Fish: Aqueous and Dietary Exposure
OECD
This Test Guideline describes a procedure for characterising the bioconcentration potential of substances in fish, using an aqueous (standard and minimised tests) or dietary exposure, under flow-through conditions (but semi-static regimes are ...
    23 July 2010 Click to Access:  Test No. 317: Bioaccumulation in Terrestrial Oligochaetes
OECD
This Test Guideline describes procedures designed to assess bioaccumulation of chemicals in soil oligochaetes. The parameters which characterise the bioaccumulation of a substance include the bioaccumulation factor (BAF), the uptake rate constant ...
    08 Sep 2009 Click to Access:  Test No. 302C: Inherent Biodegradability: Modified MITI Test (II)
OECD
This Test Guideline describes the modified MITI test (II). This test permits the measurement of the Biochemical Oxygen Demand (BOD) and the analysis of residual chemicals in order to evaluate the inherent biodegradability of chemical substances ...
    16 Oct 2008 Click to Access:  Test No. 314: Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater
OECD
This Test Guideline describes a method to assess the extent and kinetics of primary and ultimate biodegradation of organic chemicals whose route of entry into the environment begins with their discharge to wastewater. It consists of five simulation ...
    16 Oct 2008 Click to Access:  Test No. 315: Bioaccumulation in Sediment-dwelling Benthic Oligochaetes
OECD
This Test Guideline describes a method to assess bioaccumulation of sediment-associated chemicals in endobenthic oligochaetes worms. It applies to stable, neutral organic chemicals having log Kow values between 3.0 and 6.0, superlipophilic ...
    16 Oct 2008 Click to Access:  Test No. 316: Phototransformation of Chemicals in Water – Direct Photolysis
OECD
This Test guideline describes studies on phototransformation in water to determine the potential effects of solar irradiation on chemicals in surface water, considering direct photolysis only. It is designed as a tiered approach. The Tier 1 is based ...
    15 Oct 2007 Click to Access:  Test No. 313: Estimation of Emissions from Preservative - Treated Wood to the Environment
OECD
This Test Guideline is intended for testing the emissions from wood and wooden commodities that are not covered and are in contact with fresh water or seawater. Preservative treated wood test specimens are immersed in water. The recommended wood ...
    11 Sep 2006 Click to Access:  Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3
OECD
    11 July 2006 Click to Access:  Test No. 310: Ready Biodegradability - CO2 in sealed vessels (Headspace Test)
OECD
This Test Guideline is a screening method for the evaluation of ready biodegradability of chemical
    11 July 2006 Click to Access:  Test No. 311: Anaerobic Biodegradability of Organic Compounds in Digested Sludge: by Measurement of Gas Production
OECD
This Test Guideline describes a screening method for the evaluation of potential anaerobic biodegradability of organic chemicals under specific conditions. Washed digested sludge, containing low (
    23 Nov 2004 Click to Access:  Test No. 309: Aerobic Mineralisation in Surface Water – Simulation Biodegradation Test
OECD
The purpose of this test is to measure the time course of biodegradation of a test substance at low concentration in aerobic natural water and to quantify the observations in the form of kinetic rate expressions. The test is performed in batch by ...
    23 Nov 2004 Click to Access:  Test No. 312: Leaching in Soil Columns
OECD
The method described in this Test Guideline is based on soil column chromatography in disturbed soil. Two types of experiments are performed to determine (i) the leaching potential of the test substance, and (ii) the leaching potential of ...
    24 Apr 2002 Click to Access:  Test No. 307: Aerobic and Anaerobic Transformation in Soil
OECD
The method described in this Test Guideline is designed for evaluating aerobic and anaerobic transformation of chemicals in soil. The experiments are performed to determine the rate of transformation of the test substance, and the nature and rates ...
    24 Apr 2002 Click to Access:  Test No. 308: Aerobic and Anaerobic Transformation in Aquatic Sediment Systems
OECD
This Test Guideline describes a laboratory test method to assess aerobic and anaerobic transformation of organic chemicals in aquatic sediment systems. The method permits the measurement of (i) the transformation rate of the test substance in a ...
    22 Jan 2001 Click to Access:  Test No. 303: Simulation Test - Aerobic Sewage Treatment -- A: Activated Sludge Units; B: Biofilms
OECD
This Test Guideline describes two simulations tests used in the aerobic sewage treatment. Activated Sludge Units are designed to determine the elimination and the primary and/or ultimate biodegradation of water-soluble organic compounds by aerobic ...
    14 June 1996 Test No. 305: Bioconcentration: Flow-through Fish Test
OECD
This Test Guideline describes a procedure for characterising the bioconcentration potential of substances in fish, under flow-through conditions (but semi-static regimes are permissible). The test consists of two phases: the exposure (uptake) and ...
    17 July 1992 Click to Access:  Test No. 301: Ready Biodegradability
OECD
This Test Guideline describes six methods that permit the screening of chemicals for ready biodegradability in an aerobic aqueous medium. The methods are: the DOC Die-Away, the CO2 Evolution (Modified Sturm Test), the MITI (I) (Ministry of ...
    17 July 1992 Click to Access:  Test No. 302B: Inherent Biodegradability: Zahn-Wellens/ EVPA Test
OECD
This Test Guideline describes the Zahn-Wellens/EMPA Test. It is used to determine inherent biodegradability. A mixture containing the non-volatile and water soluble test substance, mineral nutrients and a relatively large amount of activated sludge ...
    17 July 1992 Click to Access:  Test No. 306: Biodegradability in Seawater
OECD
This Test Guideline describes two methods for biodegradability in seawater. The shake flask method consists on dissolution of a pre-determined amount of the test substance in the test medium to yield a concentration of 5-40 mg/l dissolved organic ...
    12 May 1981 Click to Access:  Test No. 302A: Inherent Biodegradability: Modified SCAS Test
OECD
This Test Guideline describes a method which is an adaptation of the Soap and Detergent Association semi-continuous activated sludge (SCAS) procedure for assessing the primary biodegradation of alkyl benzene sulphonate. The test does not simulate ...
    12 May 1981 Click to Access:  Test No. 304A: Inherent Biodegradability in Soil
OECD
The method described in this Test Guideline is designed for the evaluation of the mineralisation rate of a 14C-labelled compound in soil. The method is applicable to volatile or non-volatile, soluble or insoluble compounds which are not inhibitory ...
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