OECD Guidelines for the Testing of Chemicals, Section 2

Effects on Biotic Systems

Test No. 238: Sediment-Free Myriophyllum Spicatum Toxicity Test
ISSN :
2074-5761 (online)
DOI :
10.1787/20745761
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The OECD Guidelines for the Testing of Chemicals is a collection of about 100 of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of new and existing chemical substances, chemical preparations and chemical mixtures. They are a set of tools for professionals, used primarily in regulatory safety testing and subsequent chemical and chemical product notification and chemical registration. They can also be used for the selection and ranking of candidate chemicals during the development of new chemicals and products and in toxicology research. This group of tests covers effects on biotic systems.

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Mark Number Date TitleClick to Access
    26 Sep 2014 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9714491e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-238-sediment-free-myriophyllum-spicatum-toxicity-test_9789264224131-en
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Test No. 238: Sediment-Free Myriophyllum Spicatum Toxicity Test
OECD
This Test Guideline assesses the toxicity of chemicals on the growth of submerged aquatic plants Myriophyllum spicatum growing in a sediment-free test system.
    26 Sep 2014 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9714501e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-239-water-sediment-myriophyllum-spicatum-toxicity-test_9789264224155-en
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Test No. 239: Water-Sediment Myriophyllum Spicatum Toxicity Test
OECD
This Test Guideline assesses the toxicity of chemicals on the growth of the rooted aquatic plants (Myriophyllum spicatum) growing in water-sediment system.
    26 July 2013 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9713161e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-236-fish-embryo-acute-toxicity-fet-test_9789264203709-en
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Test No. 236: Fish Embryo Acute Toxicity (FET) Test
OECD
The test method described in this Test Guidelineis inteneded to determine the acute or letal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilised zebrafish eggs are exposed to the test chemical for a period of 96 hrs ...
    26 July 2013 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9713171e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-237-honey-bee-apis-mellifera-larval-toxicity-test-single-exposure_9789264203723-en
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Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure
OECD
This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical. On day 1 (D1) of the study ...
    26 July 2013 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9713191e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-210-fish-early-life-stage-toxicity-test_9789264203785-en
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Test No. 210: Fish, Early-life Stage Toxicity Test
OECD
The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...
    02 Oct 2012 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9712171e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-211-daphnia-magna-reproduction-test_9789264185203-en
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Test No. 211: Daphnia magna Reproduction Test
OECD
The test method described in this Test Guideline assesses the effect of chemicals on the reproductive output of Daphnia magna Straus. To this end, young female Daphnia are exposed to the test substance added to water at a range of concentrations (at ...
    02 Oct 2012 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9712181e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-229-fish-short-term-reproduction-assay_9789264185265-en
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Test No. 229: Fish Short Term Reproduction Assay
OECD
This Test Guideline describes an in vivo screening assay for fish reproduction where sexually mature male and spawning female fish are held together and exposed to a chemical during a limited part of their life-cycle (21 days). The short term ...
    28 July 2011 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720101e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-201-alga-growth-inhibition-test_9789264069923-en
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Test No. 201: Freshwater Alga and Cyanobacteria, Growth Inhibition Test
OECD
The purpose of this test is to determine the effects of a substance on the growth of freshwater microalgae and/or cyanobacteria. Exponentially growing test organisms are exposed to the test substance in batch cultures over a period of normally 72 ...
    28 July 2011 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723401e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-234-fish-sexual-development-test_9789264122369-en
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Test No. 234: Fish Sexual Development Test
OECD
This Test Guideline describes an assay that assesses early life-stage effects and potential adverse consequences of putative endocrine disrupting chemicals (e.g. oestrogens, androgens and steroidogenesis inhibitors) on fish sexual development. In ...
    28 July 2011 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723501e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-235-chironomus-sp-acute-immobilisation-test_9789264122383-en
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Test No. 235: Chironomus sp., Acute Immobilisation Test
OECD
This Test Guideline describes an acute immobilisation assay on chronomids and is designed to complement existing Test Guidelines for chironomid chronic toxicity assays (TG 218, 219 and 233). The test method is based on TG 202: Daphnia sp. Acute ...
    23 July 2010 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720901e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-209-activated-sludge-respiration-inhibition-test_9789264070080-en
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Test No. 209: Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)
OECD
This Test Guideline is designed to assess the effects of a substance on micro-organisms from activated sludge of waste-water treatment plants by measuring their respiration rate (carbon and/or ammonium oxidation) as oxygen consumption. The test ...
    23 July 2010 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722301e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-223-avian-acute-oral-toxicity-test_9789264090897-en
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Test No. 223: Avian Acute Oral Toxicity Test
OECD
This Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only ...
    23 July 2010 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723301e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-233-sediment-water-chironomid-life-cycle-toxicity-test-using-spiked-water-or-spiked-sediment_9789264090910-en
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Test No. 233: Sediment-Water Chironomid Life-Cycle Toxicity Test Using Spiked Water or Spiked Sediment
OECD
This Guideline is designed to assess the effects of prolonged exposure of chemicals to the life-cycle of the sediment-dwelling freshwater dipteran Chironomus sp. First instar chironomid larvae are exposed to five concentrations of the test chemical ...
    08 Sep 2009 Test No. 229: Fish Short Term Reproduction Assay
OECD
This Test Guideline describes an in vivo screening assay for fish reproduction where sexually mature male and spawning female fish are held together and exposed to a chemical during a limited part of their life-cycle (21 days). The short term ...
    08 Sep 2009 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723001e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-230-21-day-fish-assay_9789264076228-en
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Test No. 230: 21-day Fish Assay
OECD
This Test Guideline describes an in vivo screening assay for certain endocrine active substances where sexually mature male and spawning female fish are held together and exposed to a chemical during a limited part of their life-cycle (21 days ...
    08 Sep 2009 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723101e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-231-amphibian-metamorphosis-assay_9789264076242-en
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Test No. 231: Amphibian Metamorphosis Assay
OECD
This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is ...
    08 Sep 2009 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9723201e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-232-collembolan-reproduction-test-in-soil_9789264076273-en
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Test No. 232: Collembolan Reproduction Test in Soil
OECD
This Test Guideline is designed for assessing the effects of chemicals on the reproduction of collembolans in soil. The parthenogenetic Folsomia candida is the recommended species for use, but an alternative species such as sexually reproducing ...
    16 Oct 2008 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722601e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-226-predatory-mite-hypoaspis-geolaelaps-aculeifer-reproduction-test-in-soil_9789264067455-en
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Test No. 226: Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil
OECD
This Test Guideline describes a method to assess the effects of chemical substances in soil on the reproductive output of the soil mite species Hypoaspis (Geolaelaps) aculeifer Canestrini (Acari: Laelapidae). It can be used for water soluble or ...
    16 Oct 2008 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722801e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-228-determination-of-developmental-toxicity-of-a-test-chemical-to-dipteran-dung-flies-scathophaga-stercoraria-l-scathophagidae-musca-autumnalis-de-geer-muscidae_9789264067479-en
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Test No. 228: Determination of Developmental Toxicity of a Test Chemical to Dipteran Dung Flies(Scathophaga stercoraria L. (Scathophagidae), Musca autumnalis De Geer (Muscidae))
OECD
This Test Guideline describes a method to estimate the developmental toxicity of a test chemical to the dung dwelling life stages of dung-dependent dipteran species. Two test species can be used. The test chemical is mixed with bovine faeces, to ...
    16 Oct 2008 Test No. 211: Daphnia magna Reproduction Test
OECD
The test method described in this Test Guideline assesses the effect of chemicals on the reproductive output of Daphnia magna Straus. To this end, young female Daphnia are exposed to the test substance added to water at a range of concentrations (at ...
    15 Oct 2007 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722501e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-225-sediment-water-lumbriculus-toxicity-test-using-spiked-sediment_9789264067356-en
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Test No. 225: Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment
OECD
This Test Guideline is designed to assess the effects of prolonged exposure to sediment-associated chemicals on the reproduction and the biomass of the endobenthic oligochaete Lumbriculus variegatus (Müller). The method is described for static test ...
    25 Jan 2007 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722401e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-224-determination-of-the-inhibition-of-the-activity-of-anaerobic-bacteria_9789264067332-en
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Test No. 224: Determination of the Inhibition of the Activity of Anaerobic Bacteria
OECD
This Test Guideline describes a method, which can provide information that is useful in predicting the likely effect of a test substance on gas production in anaerobic digesters. Aliquots of a mixture of anaerobically digesting sludge (20 g/L to 40 ...
    11 Sep 2006 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720001e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/summary-of-considerations-in-the-report-from-the-oecd-expert-group-on-ecotoxicology_9789264022386-en
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Summary of Considerations in the Report from the OECD Expert Group on Ecotoxicology
OECD
    17 Aug 2006 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722701e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-227-terrestrial-plant-test-vegetative-vigour-test_9789264067295-en
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Test No. 227: Terrestrial Plant Test: Vegetative Vigour Test
OECD
This Test Guideline is designed to assess effects on vegetative vigour of terrestrial plants following above-ground exposure by general chemicals, biocides and crop protection products. The test can be conducted in order to determine the ...
    17 Aug 2006 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720801e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-208-terrestrial-plant-test-seedling-emergence-and-seedling-growth-test_9789264070066-en
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Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test
OECD
This Test Guideline is designed to assess effects on seedling emergence and early growth of higher plants following exposure to the test substance applied to the soil surface or into the soil. Seeds are placed in contact with soil treated with the ...
    11 July 2006 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722101e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-221-lemna-sp-growth-inhabition-test_9789264016194-en
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Test No. 221: Lemna sp. Growth Inhibition Test
OECD
This Test Guideline is designed to assess the toxicity of substances to freshwater aquatic plants of the genus Lemna (duckweed). Exponentially growing plant cultures of the genus Lemna (Lemna gibba and Lemna minor usually) are allowed to grow as ...
    23 Nov 2004 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720201e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-202-daphnia-sp-acute-immobilisation-test_9789264069947-en
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Test No. 202: Daphnia sp. Acute Immobilisation Test
OECD
This Test Guideline describes an acute toxicity test to assess effects of chemicals towards daphnids (usually Daphnia magna Staus). Young daphnids, aged less than 24 hours at the start of the test, are exposed to the test substance at a range of ...
    23 Nov 2004 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721801e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-218-sediment-water-chironomid-toxicity-using-spiked-sediment_9789264070264-en
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Test No. 218: Sediment-Water Chironomid Toxicity Using Spiked Sediment
OECD
This Test Guideline is designed to assess the effects of prolonged exposure of chemicals to the sediment-dwelling larvae of the freshwater dipteran Chironomus sp. First instar chironomid larvae are exposed to at least five concentrations of the test ...
    23 Nov 2004 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721901e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-219-sediment-water-chironomid-toxicity-using-spiked-water_9789264070288-en
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Test No. 219: Sediment-Water Chironomid Toxicity Using Spiked Water
OECD
This Test Guideline is designed to assess the effects of prolonged exposure of chemicals to the sediment-dwelling larvae of the freshwater dipteran Chironomus sp. First instar chironomid larvae are exposed to at least five concentrations of the test ...
    23 Nov 2004 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722001e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-220-enchytraeid-reproduction-test_9789264070301-en
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Test No. 220: Enchytraeid Reproduction Test
OECD
This Test Guideline is designed to be used for assessing the effects of chemicals on the reproductive output of the enchytraeid worm, Enchytraeus albidus Henle 1873, in soil. Adult enchytraeid worms are exposed to a range of concentrations of the ...
    23 Nov 2004 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9722201e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-222-earthworm-reproduction-test-eisenia-fetida-eisenia-andrei_9789264070325-en
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Test No. 222: Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)
OECD
This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei. Adult worms are exposed to a range of ...
    21 Jan 2000 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721501e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-215-fish-juvenile-growth-test_9789264070202-en
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Test No. 215: Fish, Juvenile Growth Test
OECD
This Test Guideline is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish. Juvenile fish in exponential growth phase are placed, after being weighed, in test chambers and are normally exposed to five ...
    21 Jan 2000 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721601e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-216-soil-microorganisms-nitrogen-transformation-test_9789264070226-en
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Test No. 216: Soil Microorganisms: Nitrogen Transformation Test
OECD
This Test Guideline describes a laboratory test method designed to investigate the long-term effects of chemicals, after a single exposure, on nitrogen transformation activity of soil microorganisms. Sieved soil is amended with powdered plant meal ...
    21 Jan 2000 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721701e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-217-soil-microorganisms-carbon-transformation-test_9789264070240-en
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Test No. 217: Soil Microorganisms: Carbon Transformation Test
OECD
This Test Guideline describes a laboratory test method designed to investigate long term potential effects of a single exposure of agrochemicals/non agrochemicals on carbon transformation activity of soil microorganisms. A minimum of two test ...
    21 Sep 1998 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721201e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-212-fish-short-term-toxicity-test-on-embryo-and-sac-fry-stages_9789264070141-en
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Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages
OECD
In this Short-term Toxicity Test on Fish Embryo and Sac-Fry Stages, the life stages from the newly fertilized egg to the end of the sac-fry stage are exposed. The embryo and sac-fry stages of fish are exposed to five concentrations of the test ...
    21 Sep 1998 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721301e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-213-honeybees-acute-oral-toxicity-test_9789264070165-en
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Test No. 213: Honeybees, Acute Oral Toxicity Test
OECD
This Test Guideline is a laboratory test method, designed to assess the oral acute toxicity of pesticides and other chemicals, to adult worker honeybees. Adult worker honeybees are exposed to five doses in a geometric series of the test substance ...
    21 Sep 1998 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9721401e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-214-honeybees-acute-contact-toxicity-test_9789264070189-en
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Test No. 214: Honeybees, Acute Contact Toxicity Test
OECD
This Test Guideline is a laboratory test method, designed to assess the acute contact toxicity of pesticides and other chemicals to adult worker honeybees. Anaesthetized adult worker honeybees are exposed to five doses in a geometric series of the ...
    17 July 1992 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720301e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-203-fish-acute-toxicity-test_9789264069961-en
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Test No. 203: Fish, Acute Toxicity Test
OECD
The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible. One or more species ...
    17 July 1992 Test No. 210: Fish, Early-Life Stage Toxicity Test
OECD
The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to ,at least, five concentrations of the ...
    04 Apr 1984 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720401e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-204-fish-prolonged-toxicity-test-14-day-study_9789264069985-en
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Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study
OECD
This Test Guideline presents guidance for measurement of lethal and other observed effects in fish exposed to test substances. It may be used in place of Test Guideline 203 if a longer observation period is considered useful and appropriate and the ...
    04 Apr 1984 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720501e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-205-avian-dietary-toxicity-test_9789264070004-en
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Test No. 205: Avian Dietary Toxicity Test
OECD
The purpose of this Test Guideline is to determine the effects of a substance administered with food to birds. Birds are fed a diet containing the test substance at a range of concentrations for a period of five days. Two control groups and one ...
    04 Apr 1984 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720601e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-206-avian-reproduction-test_9789264070028-en
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Test No. 206: Avian Reproduction Test
OECD
The purpose of this Test Guideline is to determine the effects on the reproduction of a substance administered with food to birds. Birds are fed a diet containing the test substance in various concentrations for a period of not less than 20 weeks. A ...
    04 Apr 1984 Click to Access: 
    http://oecd.metastore.ingenta.com/content/9720701e.pdf
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  • http://www.keepeek.com/Digital-Asset-Management/oecd/environment/test-no-207-earthworm-acute-toxicity-tests_9789264070042-en
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Test No. 207: Earthworm, Acute Toxicity Tests
OECD
This Test Guideline includes two methods: a paper contact toxicity test and an artificial soil test. The recommended specie is Eisenia foetida (Michaelsen). The initial screening test (filter paper contact test) involves exposing earthworms to test ...
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